RECOMBINANT DOG TNF ALPHA PDP001 from Bio-Rad (Formerly AbD Serotec)

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RECOMBINANT DOG TNF ALPHA

Published customer image:
Recombinant canine TNF-α (PDP001) used to stimulate canine aortic endothlial cells in vitro prior to protein extraction and western blotting.
Image caption:
Adhesion molecule protein expression in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin, P-selectin and VCAM-1 was studied by Western blotting (A); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization.

From: Grob D, Conejeros I, López-Osorio S, Velásquez ZD, Segeritz L, Gärtner U, Schaper R, Hermosilla C, Taubert A.
Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
Biology. 2021; 10(5):427.
doi: 10.3390/biology10050427
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
RECOMBINANT DOG TNF ALPHA

Published customer image:
Recombinant canine TNF-α (PDP001) used to stimulate canine aortic endothlial celly in vitro prior to protein extraction and western blotting.
Image caption:
Adhesion molecule protein expression in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin, P-selectin and VCAM-1 was studied by Western blotting (A); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization.

From: Grob D, Conejeros I, López-Osorio S, Velásquez ZD, Segeritz L, Gärtner U, Schaper R, Hermosilla C, Taubert A.
Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
.
doi: 10.3390/biology10050427
This image is from an open access article distributed under terms of a.
RECOMBINANT DOG TNF ALPHA

Published customer image:
Recombinant canine TNF-α (PDP001) used to stimulate canine aortic endothlial cells in vitro prior to protein extraction and western blotting.
Image caption:
Adhesion molecule protein expression in A. vasorum antigen (AvAg)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen (AvAg; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin, P-selectin and VCAM-1 was studied by Western blotting (A); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization.

From: Grob D, Conejeros I, López-Osorio S, Velásquez ZD, Segeritz L, Gärtner U, Schaper R, Hermosilla C, Taubert A.
Canine Angiostrongylus vasorum-Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro.
Biology. 2021; 10(5):427.
doi: 10.3390/biology10050427
This image is from an open access article distributed under terms of a Creative Commons Attribution License.